Fig 2: RT-qPCR, western blotting and immunohistochemistry analysis of Ifi44 expression in the kidney tissue. (A) RT-PCR analysis demonstrated significant upregulation of Ifi44 transcription in the GM group (*P<0.05). (B) Western blotting confirmed increased Ifi44 protein in the GM group. (C) Immunohistochemistry analysis also demonstrated upregulation of Ifi44 in the kidney tissue. Left panels, control group; right panels, GM group; upper panels, 200 µm scale bar; bottom panels, 50 µm scale bar. RT-qPCR, reverse transcription-quantitative polymerase reaction; Ctrl, control; GM, gentamicin; Ifi44, interferon-inducible protein 44.